1. Delineating the mutational effects on multiple RNA conformations
How do mutations affect RNA functions, especially those that are structurally dynamic? I recently showed in a dynamic self-splicing intron that mutations affecting the formation of multiple conformational states can be delineated effectively using deep mutational scanning (DMS). This approach has allowed the quantification of the mutational effects in a combinatorial manner, and critically, the construction of a fitness landscape for the self-splicing intron.
Keywords: Fitness landscape, genotype-phenotype relationships, deep mutational scanning, RNA, self-splicing intron